To Determine The pH Value of Water Sample Using Indicator Dyes
Reagents: Universal Indicator, Bromothymol Lee Indicator
Apparatus: Test Tues, Stirrer, Aquascope
Theory: The pH Value of Water is defined as the log of reciprocal of hydrogen ions present in that water. It represents acidity or alkalinity in water. The maximum acidity will be at 0 value of pH and the maximum alkalinity will be at pH Value of 14.
Procedure:
I. Take four test tubes and fill them one fourth each with sample water.
II. Add 15 drops universal indicator reagent to each of the four test tubes.
III. Mix the solution thoroughly.
IV. Observe the tinge of colors developed in the test tubes and match them with the colour scale given on the indicator bottle.
V. Water Sample found to have in range of 6 - 7.6 in the above test are further subjected to testing by Bromothymol Blue Indicator in an Aquascope.
VI. Fill the sample to be tested in the cell upto block line mark.
VII. Put 15 drops of Bromothymol Blue Indicator in middle compartment of the cell, and stir it with a Stirrer. Wait for 5 minutes.
VIII. A colour will develop.
IX. Match the colour with the standard colour slides available in Aquascope itself. The two slides are put in the slots in front of the slide compartments of the cell and then the colour developed in the middle compartment is matched with the slides on the sides.
II. Add 15 drops universal indicator reagent to each of the four test tubes.
III. Mix the solution thoroughly.
IV. Observe the tinge of colors developed in the test tubes and match them with the colour scale given on the indicator bottle.
V. Water Sample found to have in range of 6 - 7.6 in the above test are further subjected to testing by Bromothymol Blue Indicator in an Aquascope.
VI. Fill the sample to be tested in the cell upto block line mark.
VII. Put 15 drops of Bromothymol Blue Indicator in middle compartment of the cell, and stir it with a Stirrer. Wait for 5 minutes.
VIII. A colour will develop.
IX. Match the colour with the standard colour slides available in Aquascope itself. The two slides are put in the slots in front of the slide compartments of the cell and then the colour developed in the middle compartment is matched with the slides on the sides.
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